Advances in Microbial Ecology by Douglas E. Caldwell, Darren R. Korber, John R. Lawrence

By Douglas E. Caldwell, Darren R. Korber, John R. Lawrence (auth.), K. C. Marshall (eds.)

Advances in Microbial Ecology was once demonstrated via the overseas Committee on Microbial Ecology (ICOME) to supply a motor vehicle for in-depth, serious, or even provocative reports to stress fresh traits within the vital box of microbial ecology. Advances in Microbial Ecology is now famous as a tremendous resource of infor­ mation and thought either for training and for potential microbial ecologists. such a lot stories showing in Advances were ready by way of leaders particularly components stick to­ ing invites issued via the Editorial Board. people are inspired, although, to publish outlines of unsolicited contributions to any member of the Editorial Board for attention for ebook in Advances. With the e-book of quantity 12 of Advances in Microbial Ecology there'll be a metamorphosis of Editor and the whole Editorial Board. the present Editor needs to take this chance to thank the current Editorial Board, Ron Atlas, Bo Barker J~rgensen, and Gwyn Jones, in addition to earlier contributors of the Board, for his or her advice and inspire­ ment through the years. the hot Editor of Advances in Microbial Ecology might be Gwyn Jones, with Bernhard Schink, Warwick F. Vincent, and David M. Ward as individuals of the Editorial Board. The outgoing Board want the recent Board each luck in continu­ ing the traditions tested by means of Martin Alexander, the founding Editor of Advances in Microbial Ecology. the subjects featured in quantity 12 of Advances contain a few on the topic of the meta­ bolic actions of micro organism; specifically, bioremediation of oil spills, through R. M. Atlas and R.

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1989a), the ability of polarly flagellated, motile and nonmotile P. 2 IJ-m of the surface, respectively). Motile P. fluorescens actively moved upstream from the biofilm "front" against high flow even though the maximum rate of motility for this strain was 85 IJ-m/sec. This resulted in backgrowth (upstream colonization) exceeding that which could have occurred from growth alone, as confirmed by observing a nonmotile P. fluorescens mutant which was unable to backgrow (Fig. 14). Thus, motile attachment due to flagellar rotation is responsible, in part, for bacterial positioning within flowing systems.

6. This latter approach has not yet been applied in microbial ecology. 2. Difference Imaging of Growth Bacterial growth has been directly visualized by using difference imagery (Caldwell and Germida, 1985). This method can be used to discriminate growing cells from dead cells, fine sand, clay particles, and other debris to obtain a more accurate direct viable count and to calculate the rate of cell growth. The image of bacterial cells is digitized at time zero and after a short time interval. By subtracting the first image from the second, a difference image is constructed showing only the differences between the two original images, and removing any objects which remain unchanged.

1. Strengths and Weaknesses of Confocal Laser Imaging In conventional light microscopy, all light passing through the specimen is imaged directly and simultaneously. The resulting image is formed instantly and can be viewed directly. However, these conventional images lack clarity and fail to accurately represent 3-D objects. Stray light from above, below, and beside an object interferes with formation of the object's image. In CLM, the confocal pinholes at the laser and at the detector eliminate these sources of interference.

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